The mass spectrometry facility at the JHU Chemistry Department contains 4 different instruments equipped with a variety of different ionization techniques. Each is based on a different combination of mass analyzer design and ionization source and therefore has different analytical capabilities. Together, they form a suite of instruments that provides a comprehensive range of analytical organic mass spectral techniques. The four instruments are: VG Analytical VG-70S Magnetic Sector Mass Spectrometer (Remsen B.13) The VG-70S is a high resolution, double focusing magnetic sector (BE geometry) instrument with an effective mass range of 1-2000Da at full acceleration voltage and is equipped with EI, CI and FAB ionization methods. It is primarily used to provide both full scan nominal-mass spectra and accurate mass determinations of small molecules. The instrument is operated on a service basis and is located in Room B13, Remsen Hall. Thermo Finnigan LCQ Deca Ion Trap Mass Spectrometer (Remsen B.14) The LCQ is an ion-trap based instrument normally fitted with an electrospray (ESI) ionization source. It is primarily used for analysis of bio-molecules in solution such as proteins, peptides, biopolymers and oligonucleotides. This instrument is capable of performing multi-stage ion fragmentation analyses (MSn), making it extremely useful for structural characterization of delicate bio-molecules. It can be operated on a stand-alone basis or integrated with a Finnigan Surveyor LC system for LCMS applications. Operation is on a “self-service” basis by users following training and “check-out”. This instrument is located in Room B14, Remsen Hall. Shimadzu GC17A/QP5050A GCMS (Remsen B.14) This instrument is used for routine mass spectral analysis of volatile organic mixtures. It consists of an autosampler equipped capillary GC integrally connected with an EI ionization quadrupole mass analyzer (CI is available as an option). A low polarity (5% phenyl-, 95%methyl-siloxane) column is normally installed. This instrument is also operated on a “self-service” basis by users following training and “check-out”. It is located in Room B14, Remsen Hall. Kratos Kompact SEQ MALDI-TOF (Remsen B.06) The SEQ is a MALDI-TOF mass spectrometer used for qualitative analysis of high mass “delicate” (>70kDa) bio-molecules that cannot be analyzed by FAB or ESI-MS. It is equipped with a 337nm pulsed UV laser, a 1.7m length flight tube and a curved field reflectron and can analyze both positive and negative ion species. It is typically operated in the linear mode, though reflectron and post source decay (PSD) modes are also available if required. This instrument is operated by users on a “self-service” basis following training and “check-out”, and is located in Room B06, Remsen Hall. | 
